John Bromfield

PEER REVIEWED PUBLICATIONS

A full list of publications can be found here.

Tariq A, Sagheer M, Seekford ZK, Ren J, Yue F, Bromfield JJ. Heat shock suppresses the innate immune response of bovine endometrial epithelial cells. Reprod Fertil. 2026 Feb 19;7(1):RAF250213.

Devkota I, Bonomo ZL, Fuego DM, Li Y, Zhang X, Loux SC, Looney CR, Maia AIV, Donnarumma F, Matsakas A, Vourekas A, Elzer PH, Fu X, Bondioli KR, Bromfield JJ, Bermejo-Álvarez P, Simintiras CA. Establishment and Functional Characterization of Bovine Endometrial Epithelial Organoids. FASEB J. 2026 Feb 28;40(4):e71515.

Cao D, Ammad M, Subhadra B, Panda MK, Inzana TJ, Cunha F, Casaro S, Jones KL, Ramirez-Hernandez R, Bromfield JJ, Galvão KNA, Jeon SJ. The interactive relationship between Fusobacterium necrophorum, Bacteroides pyogenes, and Porphyromonas levii in driving inflammatory uterine disease. Microbiome. 2026 Jan 24;14(1):69.

Seekford ZK, Tariq A, Haimon MLJ, Hoorn QA, Macay GA, Zhai Y, Block J, Rabaglino MB, Jeong KC, Hansen PJ, Bromfield JJ. Induced uterine infection alters bovine endometrial function and embryonic competence. Reproduction. 2026 Feb 5;171(2):xaaf027.

Maldonado MBC, de Lima CB, Oudihat M, Block J, Sagheer M, Cuellar CJ, Yu F, Zhang Y, Haimon MLJ, Bromfield JJ, Sirard MA, Hansen PJ. Alterations in gene expression and DNA methylation in the bovine blastocyst in response to choline supplementation during culture. Biol Reprod. 2026 May 13;114(5):1568-1581.

Macay GA, Gonzalez TD, Seekford ZK, Ramirez-Hernandez R, Molinari PCC, Dickson MJ, Harstine BR, Block J, Bromfield JJ. Seminal plasma alters the endometrium during early embryo development in cattle. Biol Reprod. 2026 May 13;114(5):1635-1645.

Fontes PLP, Davis DB, Melo-Gonçalves L, Burato S, Smith MS, Stelzleni A, Fluharty FF, Stewart RL Jr, Cooke RF, Bromfield JJ, Else-Keller A, Kerns K, Strickland L, Zoca SM. Overnutrition induced metabolic dysregulation and partially decreased semen quality in young beef bulls. J Anim Sci. 2026 Jan 8;104:skag004.

Tariq A, Melo-Gonçalves L, Maia TS, Hoorn QA, Davis DB, Zoca SM, Stewart RL Jr, Fontes PLP, Bromfield JJ. Overnutrition of young bulls delays in vitro embryonic development and decreases embryo quality. Biol Reprod. 2026 Jan 11;114(1):102-115.

Lindner EE, Bonney-King J, Burner CM, Krawczel PD, Bromfield JJ, Bittar JHJ, Martins T, Miller-Cushon EK. Long-term effects of preweaning social housing on growth and reproductive development of dairy heifers. J Dairy Sci. 2025 Sep;108(9):10023-10036.

Seekford ZK, Wohlgemuth SE, Sheldon IM, Bromfield JJ. Exposure of bovine granulosa cells to lipopolysaccharide reduces progesterone secretion during luteinization. Biol Reprod. 2025 Jun 15;112(6):1243-1255.

Fontes PLP, Bromfield JJ, Pohler KG, Lamb GC. Impact of paternal high energy diets on semen quality and embryo development in cattle. Reprod Fertil. 2025 Feb 17;6(1):e240082.

Tariq A, Seekford ZK, Bromfield JJ. Inflammation during oocyte maturation reduces developmental competence and increases apoptosis in blastocysts. Biol Reprod. 2025 Mar 16;112(3):420-433.

Seekford ZK, Tariq A, Macay GA, Jenkins KM, Dickson MJ, Melo GD, Pohler KG, Sheldon IM, Bromfield JJ. Uterine disease in dairy cows is associated with contemporaneous perturbations to ovarian function. Theriogenology. 2025 Jan 15;232:20-29.

Mirzaei A, Londoño-Mendez MC, Lasso-Ramirez S, Adams PE, Seekford ZK, Bromfield JJ, Heredia D, Di Lorenzo N, Chebel RC, Gonella-Diaza AM. Embryo production by Holstein heifers superovulated with a recombinant long-acting follicle-stimulating hormone analog. J Anim Sci. 2024 Jan 3;102:skae326.

Bromfield JJ. Interaction of semen with female reproductive tract tissues: what we know, what we guess and what we need to do. Anim Reprod. 2024 Aug 5;21(3):e20240042.

Fritsche KLM, Ahola JK, Pinedo PJ, Seidel GE, Rhoades RD, Stevenson JS, Olson KC, Jaeger JR, Grieger DM, Bromfield JJ. Pregnancy risk in beef and dairy cows after supplementing semen with transforming growth factor beta-1 at the time of artificial insemination. J Anim Sci. 2024 Jan 3;102:skae169.

Peixoto PM, Bromfield JJ, Ribeiro ES, Santos JEP, Thatcher WW, Bisinotto RS. Transcriptome changes associated with elongation of bovine conceptuses II: Differentially expressed transcripts in the endometrium on day 17 after insemination. J Dairy Sci. 2023 Dec;106(12):9763-9777.

Peixoto PM, Bromfield JJ, Ribeiro ES, Santos JEP, Thatcher WW, Bisinotto RS. Transcriptome changes associated with elongation of bovine conceptuses I: Differentially expressed transcripts in the conceptus on day 17 after insemination. J Dairy Sci. 2023 Dec;106(12):9745-9762.

Husnain A, Arshad U, Zimpel R, Schmitt E, Dickson MJ, Perdomo MC, Marinho MN, Ashrafi N, Graham SF, Bishop JV, Hansen TR, Jeong KC, Gonella-Diaza AM, Chebel RC, Sheldon IM, Bromfield JJ, Santos JEP. Induced endometrial inflammation compromises conceptus development in dairy cattle. Biol Reprod. 2023 Oct 13;109(4):415-431.

Molinari PCC, Bromfield JJ. Inflammatory responses of bovine endometrial epithelial cells are increased under in vitro heat stress conditions. J Therm Biol. 2023 May;114:103564.

Seekford ZK, Davis DB, Dickson MJ, Melo Gonçlaves L, Burato S, Holton MP, Gordon J, Pohler KG, Cliff Lamb G, Pringle TD, Stewart RL, Ferrer MS, Fontes PLP, Bromfield JJ. Bulls fed a high-gain diet decrease blastocyst formation after in vitro fertilization. Reproduction. 2023 Jul 3;166(2):149-159.

Husnain A, Arshad U, Poindexter MB, Zimpel R, Marinho MN, Perdomo MC, Fan P, Jeong KC, Nelson CD, Sheldon IM, Bromfield JJ, Santos JEP. Induced endometritis in early lactation compromises production and reproduction in dairy cows. J Dairy Sci. 2023 Jun;106(6):4198-4213.

Ormsby TJR, Owens SE, Turner ML, Cronin JG, Bromfield JJ, Sheldon IM. Glucocorticoids increase tissue cell protection against pore-forming toxins from pathogenic bacteria. Commun Biol. 2023 Feb 17;6(1):186.

Molinari PCC, Davidson BD, Laporta J, Dahl GE, Sheldon IM, Bromfield JJ. Prepartum heat stress in dairy cows increases postpartum inflammatory responses in blood of lactating dairy cows. J Dairy Sci. 2023 Feb;106(2):1464-1474.

Rocha CC, Silva FAC, Martins T, Marrero MG, Bromfield JJ, Driver J, Hansen T, Oliveira L, Binelli M. Culture of endometrial epithelial cells collected by a cytological brush in vivo. JDS Commun. 2022 Mar 16;3(3):217-221.

Molinari PCC, Dahl GE, Sheldon IM, Bromfield JJ. Effect of calving season on metritis incidence and bacterial content of the vagina in dairy cows. Theriogenology. 2022 Oct 1;191:67-76.

Horlock AD, Ormsby TJR, Clift MJD, Santos JEP, Bromfield JJ, Sheldon IM. Cholesterol supports bovine granulosa cell inflammatory responses to lipopolysaccharide. Reproduction. 2022 Aug 5;164(3):109-123.

Dickson MJ, Bishop JV, Hansen TR, Sheldon IM, Bromfield JJ. The endometrial transcriptomic response to pregnancy is altered in cows after uterine infection. PLoS One. 2022 Mar 31;17(3):e0265062.

Ormsby TJR, Owens SE, Clement L, Mills TJ, Cronin JG, Bromfield JJ, Sheldon IM. Oxysterols Protect Epithelial Cells Against Pore-Forming Toxins. Front Immunol. 2022 Jan 26;13:815775.

Dickson MJ, Sheldon IM, Bromfield JJ. Lipopolysaccharide alters CEBPβ signaling and reduces estradiol production in bovine granulosa cells. CABI Agric Biosci. 2022;3(1):66.

Molinari PCC, Nally JE, Bromfield JJ. Bovine endometrial cells do not mount an inflammatory response to Leptospira. Reprod Fertil. 2021;2(3):187-198.

Ormsby TJR, Owens SE, Horlock AD, Davies D, Griffiths WJ, Wang Y, Cronin JG, Bromfield JJ, Sheldon IM. Oxysterols protect bovine endometrial cells against pore-forming toxins from pathogenic bacteria. FASEB J. 2021 ;35(10):e21889.

Horlock AD, Ormsby TJR, Clift MJD, Santos JEP, Bromfield JJ, Sheldon IM. Manipulating bovine granulosa cell energy metabolism limits inflammation. Reproduction. 2021; 161(5):499-512.

Vyas D, Nelson CD, Bromfield JJ, Liyanamana P, Krause M, Dahl GE. MILK Symposium review: Identifying constraints, opportunities, and best practices for improving milk production in market-oriented dairy farms in Sri Lanka. J Dairy Sci. 2020; 103(11):9774-9790.

Edelhoff INF, Pereira MHC, Bromfield JJ, Vasconcelos JLM, Santos JEP. Inflammatory diseases in dairy cows: Risk factors and associations with pregnancy after embryo transfer. J Dairy Sci. 2020; 103(12):11970-11987.

Zolini AM, Block J, Rabaglino MB, Rincon G, Hoelker M, Bromfield JJ, Salilew-Wondim D, Hansen PJ. Genes associated with survival of female bovine blastocysts produced in vivo. Cell Tissue Res. 2020; 382(3):665-678.

Piersanti RL, Block J, Ma Z, Jeong KC, Santos JEP, Yu F, Sheldon IM, Bromfield JJ. Uterine infusion of bacteria alters the transcriptome of bovine oocytes. FASEB Bioadv. 2020;2(8):506-520.

Dickson MJ, Piersanti RL, Ramirez-Hernandez R, de Oliveira EB, Bishop JV, Hansen TR, Ma Z, Jeong KC, Santos JEP, Sheldon IM, Block J, Bromfield JJ. Experimentally induced endometritis impairs the developmental capacity of bovine oocytes. Biol Reprod. 2020; 103(3):508-520.

Horlock AD, Piersanti RL, Ramirez-Hernandez R, Yu F, Ma Z, Jeong KC, Clift MJD, Block J, Santos JEP, Bromfield JJ, Sheldon IM. Uterine infection alters the transcriptome of the bovine reproductive tract three months later. Reproduction. 2020; 160(1):93-107.

Akter S, Xu D, Nagel SC, Bromfield JJ, Pelch KE, Wilshire GB, Joshi T. GenomeForest: An ensemble machine learning classifier for endometriosis. AMIA Jt Summits Transl Sci Proc. 2020; 2020:33-42.

Sheldon IM, Molinari PCC, Ormsby TJR, Bromfield JJ. Preventing postpartum uterine disease in dairy cattle depends on avoiding, tolerating and resisting pathogenic bacteria. Theriogenology. 2020; 150:158-165.

Zolini AM, Block J, Rabaglino MB , Tribulo P, Hoelker M, Rincon G, Bromfield JJ, Hansen PJ. Molecular fingerprint of female bovine embryos produced in vitro with high competence to establish and maintain pregnancy. Biol Reprod. 2020; 102(2):292-305.

Piersanti RL, Santos JEP, Sheldon IM, Bromfield JJ. Lipopolysaccharide and tumor necrosis factor-alpha alter gene expression of oocytes and cumulus cells during bovine in vitro maturation. Mol Reprod Dev. 2019; 86(12):1909-1920.

Rizo JA, Ibrahim LA, Molinari PCC, Harstine B, Piersanti RL, Bromfield JJ. Effect of seminal plasma or transforming growth factor on bovine endometrial cells. Reproduction. 2019; 158(6):529-541.

Ortiz WG, Rizo JA, Carvalheira LR, Ahmed BMS, Estrada-Cortes E, Harstine BR, Bromfield JJ, Hansen PJ. Effects of intrauterine infusion of seminal plasma at artificial insemination on fertility of lactating Holstein cows. J Dairy Sci. 2019; 102(7):6587-6594.

Piersanti RL, Horlock AD, Block J, Santos JEP, Sheldon IM, Bromfield JJ. Persistent effects on bovine granulosa cell transcriptome after resolution of uterine disease. Reproduction. 2019; 158(1):35-46.

Piersanti RL, Zimpel R, Molinari PCC, Dickson MJ, Ma Z, Jeong KC, Santos JEP, Sheldon IM, Bromfield JJ. A model of clinical endometritis in Holstein heifers using pathogenic Escherichia coli and Trueperella pyogenes. J Dairy Sci. 2019; 102(3):2686-2697.

Sheldon IM, Cronin JG, Bromfield JJ. Tolerance and innate immunity shape the development of postpartum uterine disease and the impact of endometritis in dairy cattle. Annu Rev Anim Biosci. 2019; 7:361-384.

Ibrahim LA, Rizo JA, Fontes PLP, Lamb GC, Bromfield JJ. Seminal plasma modulates expression of endometrial inflammatory meditators in the bovine. Biol Reprod. 2019; 100(3):660-671.

Akter S, Xu D, Nagel SC, Bromfield JJ, Pelch K, Wilshire GB, Joshi T. Machine learning classifiers for endometriosis using transcriptomics and methylomics data. Front Genet. 2019; 10:766.

Pech-Cervantes AA, Ogunade IM, Jiang Y, Irfan M, Arriola KG, Amaro FX, Gonzalez CF, DiLorenzo N, Bromfield JJ, Vyas D, Adesogan AT. An expansin-like protein expands forage cell walls and synergistically increases hydrolysis, digestibility and fermentation of livestock feeds by fibrolytic enzymes. PLos One. 2019; 14(11):e0224381.

Bromfield JJ, Watt MM, Iacovides SM. Characterisation of peripheral blood mononuclear cell populations in periparturient dairy cows that develop metritis. Vet Immunol Immunopathol. 2018; 200:69-75.

Bromfield JJ. The potential of seminal fluid mediated paternal-maternal communication to optimise pregnancy success. Animal. 2018; 12(S1):104-109.

Bromfield JJ, Iacovides SM. Evaluating lipopolysaccharide-induced oxidative stress in bovine granulosa cells. J Assist Reprod Genet. 2017; 34(12):1619-1626.

Bromfield JJ, Rizo JA, Ibrahim LA. Paternal priming of maternal tissues to optimise pregnancy success. Reprod Fertil Dev. 2017; 30(1):50-55.

Bromfield JJ. A role for seminal plasma in modulating pregnancy outcomes in domestic species. Reproduction. 2016; 156(6):R223-232.

Ibrahim LA, Kramer JM, Williams RS, Bromfield JJ. Human granulosa–luteal cells initiate an innate immune response to pathogen-associated molecules. Reproduction. 2016; 152(4):261-270.

Bromfield JJ, Santos JEP, Block J, Williams SR, Sheldon IM. Uterine infection: Linking infection and innate immunity with infertility in the high producing dairy cow. J Animal Sci. 2015; 93(5):2021-2033.

Sheldon IM, Cronin JG, Healey GD, Gabler C, Heuwieser W, Streyl D, Bromfield JJ, Miyamoto A, Fergani C, Dobson H. Innate immunity and inflammation of the bovine female reproductive tract in health and disease. Reproduction. 2014; 148(3):R41-51.

Bromfield JJ. Seminal Fluid and Reproduction: Much more than previously thought. J Assist Reprod Genetics. 2014; 31(6):627-636.

Bromfield JJ, Schjenken JE, Chin PY, Care AS, Jasper MJ, Robertson SA. Maternal tract factors contribute to paternal seminal fluid impact on metabolic phenotype in offspring. Proc Natl Acad Sci USA. 2014; 111(6):2200-2205.

Price JC, Bromfield JJ, Sheldon IM. Pathogen-associated molecular patterns initiate inflammation and perturb the endocrine function of bovine granulosa cells from ovarian dominant follicles via TLR2 and TLR4 pathways. Endocrinology. 2013; 154(9):3377-3386.

Nagel SC, Bromfield JJ. Bisphenol a: a model endocrine disrupting chemical with a new potential mechanism of action. Endocrinology. 2013; 154(6):1962-1964.

Bromfield JJ, Sheldon IM. Lipopolysaccharide reduces the primordial follicle pool in the bovine ovarian cortex ex vivo and in the murine ovary in vivo. Biol Reprod. 2013; 88(4):98.

Sheldon IM, Bromfield JJ. Innate immunity in the human endometrium and ovary. Am J Reprod Immunol. 2011; 66 (Suppl 1): 63-71.

Guerin LR, Moldenhauer LM, Prins JR, Bromfield JJ, Hayball JD, Robertson SA. Seminal fluid regulates accumulation of FOXP3+ regulatory T cells in the preimplantation mouse uterus through expanding the FOXP3+ cell pool and CCL19-mediated recruitment. Biol Reprod. 2011; 85(2):397-408.

Bromfield JJ, Sheldon IM. Lipopolysaccharide initiates inflammation in bovine granulosa cells via the TLR4 pathway and perturbs oocyte meiotic progression in vitro. Endocrinology. 2011; 152(12):5029-40.

Sheldon IM, Rycroft AN, Dogan B, Craven M, Bromfield JJ, Chandler A, Roberts MH, Price SB, Gilbert RO, Simpson KW. Specific strains of Escherichia coli are pathogenic for the endometrium of cattle and cause pelvic inflammatory disease in cattle and mice. PLoS One. 2010; 5(2):e9192.

McLaughlin M, Bromfield JJ, Albertini DF, Telfer EE. Activin promotes follicular integrity and oogenesis in cultured pre-antral bovine follicles. Mol Hum Reprod. 2010; 16(9):644-53.

Coticchio G, Sciajno R, Hutt K, Bromfield J, Borini A, Albertini DF. Comparative analysis of the metaphase II spindle of human oocytes through polarized light and high-performance confocal microscopy. Fertil Steril. 2010; 93(6):2056-64.

Robertson SA, Guerin LR, Bromfield JJ, Branson KM, Ahlstrom AC, Care AS. Seminal fluid drives expansion of the CD4+ CD25+ T regulatory cell pool and induces tolerance to paternal alloantigens in mice. Biol Reprod. 2009; 80(5):1036-45.

Coticchio G, Bromfield JJ, Sciajno R, Gambardella A, Scaravelli G, Borini A, Albertini DF. Vitrification may increase the rate of chromosome misalignment in the metaphase-II spindle of human mature oocytes. Reprod Biomed Online. 2009; 19(Suppl 3):29-34.

Bromfield JJ, Coticchio G, Hutt K, Sciajno R, Borini A, Albertini DF. Meiotic spindle dynamics in human oocytes following slow-cooling cryopreservation. Hum Reprod. 2009; 24(9):2114-23.

Bromfield J, Messamore W, Albertini DF. Epigenetic regulation during mammalian oogenesis. Reprod Fertil Dev. 2008; 20(1):74-80.

Johansson M, Bromfield JJ, Jasper MJ, Robertson SA. Semen activates the female immune response during early pregnancy in mice. Immunology. 2004; 112(2):290-300.

Robertson SA, Bromfield JJ, Tremellen KP. Seminal 'priming' for protection from pre-eclampsia– A unifying hypothesis. J Reprod Immunol. 2003; 59(2):253-265.

BOOK CHAPTERS

Bromfield JJ and Piersanti RL. Mammalian Oogenesis – The fragile foundation of the next generation. In: The Ovary, 3rd Edition (2018), Leung and Adashi (Ed). Elsevier (Amsterdam, Netherlands). pp, 157-162.

Bromﬁeld JJ, Ibrahim LA, Rizo JA. Seminal Vesicle Gland—Overview. In: Encyclopedia of Reproduction (2018), M. K. Skinner (Ed). Academic Press: Elsevier. Vol. 1, pp. 341–343.

Sheldon IM, Price JC, Turner ML, Bromfield JJ, Cronin JG. Uterine infection and immunity in cattle. In: Reproduction in Domestic Ruminants VIII (2014), R. Webb (Ed). Nottingham University Press (Nottingham, UK) pp 415-430.

Bromfield JJ and Albertini DF. Soma-germline interactions: an evolutionary perspective. In: Oogenesis - The Universal Process of Oogenesis (2010), M-H. Verlhac (Ed.) Wiley-Blackwell Inc. (Oxford UK) pp 105-115.

Bromfield JJ, Jones KL, Albertini DF. In vitro maturation of mammalian oocytes. In: Biennial Review of Infertility (2009), D. Carrell, C. Racowsky, P. Schlegel and B. Van Voorhis (Eds.) Humana Press (New York NY) pp 215-222.

Robertson SA, Bromfield JJ, Glynn DJ, Sharkey DJ, Jasper MJ. Actions of seminal plasma cytokines in priming female reproductive tract receptivity for embryo implantation. In: Immunology of Implantation, (2006), Gil Mor (Ed). Landes Bioscience (Georgetown TX) pp 148-158.