RESEARCH APPROACH
The Bromfiled laboratory utilises a range of molecular and cellular techniques developed by various collaborative research scientists at the University of Florida and around the world, in addition to those developed in-house.
The University of Florida offers a unique opportunity for the Bromfield laboratory to investigate basic science using in vitro cell culture technology and conduct applied research using the Department of Animal Sciences extensive large animal resources; Dairy Research Unit, Beef Research Unit, Meats Lab and Horse Teaching Unit.
Resources within the Bromfield laboratory house state-of-the-art cell culture facilities, equipment for gene expression analysis, protein analysis and various histology, immuno-histochemical & microscopy techniques.
With these techniques and resources in addition to the dedicated, highly trained staff of scientists, graduate and undergraduate students, the laboratory makes a priority of publication in highly ranked peer-review journals, communication to the scientific & agricultural communities and acquisition of extramural funding from federal agencies.
We propose that activation of the innate immune system in response to bacterial components alters the developmental environment of the oocyte, compromising developmental competence and reducing fertility.
Bromfield et al. J Anim Sci. 2015 May;93(5):2021-33.